Gram Pos Short Essay

Gram Pos Short Essay-62
This is a mixed culture of Gram negative Escherichia coli (red-orange) and Gram positive Staphylococcus aureus (blue-purple) stained using the Gram staining method.

This is a mixed culture of Gram negative Escherichia coli (red-orange) and Gram positive Staphylococcus aureus (blue-purple) stained using the Gram staining method.

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When bacteria is properly stained, observe your slide under a microscope and record your observations. It was very small, only one micrometer in length and the results of the KOH test were fluid and watery.

The next stain I looked at was Figure 1.1, the Unknown Y bacteria which I also viewed under 1000X magnification.

Title: Identification of Two Unknown Bacteria’s Through the Use of Gram Staining Introduction: The Gram staining of bacteria is a widely used method to identify unknown bacteria.

The purpose of this method is to find out identities of unidentified bacteria.

Peptidoglycan is a macromolecule composed of sugars and amino acids that are assembled structurally like woven material.

The amino sugar component consists of alternating molecules of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM).Gram positive bacteria have cell walls composed mostly of a substance unique to bacteria known as peptidoglycan, or murein. Gram negative bacteria have cell walls with only a thin layer of peptidoglycan and an outer membrane with a lipopolysaccharide component not found in Gram positive bacteria.Gram negative bacteria stain red or pink after Gram staining.Both bacteria Y and G’s full results can be seen in Table 2.1.It shows a visual representation of how these bacteria compare in the various tests they went through.The Gram staining method, developed by Hans Christian Gram, identifies bacteria based upon the reaction of their cell walls to certain dyes and chemicals.The differences between Gram positive vs Gram negative bacteria are primarily related to their cell wall composition.These molecules are crosslinked together by short peptides which help give peptidoglycan strength and structure.Peptidoglycan provides protection for bacteria and defines their shape.It had a defined bacilli shape and ended up red after the Gram staining.This bacteria was twice as large as bacteria G, and its KOH result was a gooey suspension.

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